Nope. The car that John & Dean and Baby!Sammy were sitting on in the opening scene was the Impala. It's funny now that none of us noticed...
Also, I think Jess was an art major. There were paints and an easel and painting supplies in the background in the bedroom scene.
Oh, and what's with the lipstick and eyeliner? What girl voluntarily goes to bed wearing makeup? Even if she did fall asleep drunk she probably would have washed it off when Sam and Dean went outside to talk.
In the scene on the bridge when Dean is covered in mud, he actually sits on the Impala. Now we all know that Dean would never do that to the Impala, so my only thought is that it's an accident...
Anyway, I think I'm gonna have to make some icons of the pilot, that ep is very iconable. :)